Detection of endometrial cancer via molecular analysis of DNA collected with vaginal tampons.

ABSTRACT:

Objective

We demonstrate the feasibility of detecting EC by combining minimally-invasive specimen collection techniques with sensitive molecular testing.

Methods

Prior to hysterectomy for EC or benign indications, women collected vaginal pool samples with intravaginal tampons and underwent endometrial brushing. Specimens underwent pyrosequencing for DNA methylation of genes reported to be hypermethylated in gynecologic cancers and recently identified markers discovered by profiling over 200 ECs. Methylation was evaluated individually across CpGs and averaged across genes. Differences between EC and benign endometrium (BE) were assessed using two-sample t-tests and area under the curve (AUC).

Results

Thirty-eight ECs and 28 BEs were included. We evaluated 97 CpGs withi

SUBMITTER: Bakkum-Gamez JN 

PROVIDER: S-EPMC4380654 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature