Temporal heterogeneity in single-cell gene expression and mechanical properties during adipogenic differentiation.

ABSTRACT: Adipose-derived stem/stromal cells (ASCs) respond heterogeneously when exposed to lineage-specific induction medium. Variable responses at the single-cell level can be observed in the production of lineage-specific metabolites, expression of mRNA transcripts, and adoption of mechanical phenotypes. Understanding the relationship between the biological and mechanical characteristics for individual ASCs is crucial for interpreting how cellular heterogeneity affects the differentiation process. The goal of the current study was to monitor the gene expression of peroxisome proliferator receptor gamma (PPARG) in adipogenically differentiating ASC populations over two weeks, while also characterizing the expression-associated mechanical properties of individual cells using atomic force microscopy

SUBMITTER: Labriola NR 

PROVIDER: S-EPMC4380682 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature