T160‐phosphorylated CDK2 defines threshold for HGF dependent proliferation in primary hepatocytes.

ABSTRACT: Liver regeneration is a tightly controlled process mainly achieved by proliferation of usually quiescent hepatocytes. The specific molecular mechanisms ensuring cell division only in response to proliferative signals such as hepatocyte growth factor (HGF) are not fully understood. Here, we combined quantitative time-resolved analysis of primary mouse hepatocyte proliferation at the single cell and at the population level with mathematical modeling. We showed that numerous G1/S transition components are activated upon hepatocyte isolation whereas DNA replication only occurs upon additional HGF stimulation. In response to HGF, Cyclin:CDK complex formation was increased, p21 rather than p27 was regulated, and Rb expression was enhanced. Quantification of protein levels at the restriction poin

SUBMITTER: Mueller S 

PROVIDER: S-EPMC4380929 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature