IRNA-seq: computational method for genome-wide assessment of acute transcriptional regulation from total RNA-seq data.

ABSTRACT: RNA-seq is a sensitive and accurate technique to compare steady-state levels of RNA between different cellular states. However, as it does not provide an account of transcriptional activity per se, other technologies are needed to more precisely determine acute transcriptional responses. Here, we have developed an easy, sensitive and accurate novel computational method, IRNA-SEQ: , for genome-wide assessment of transcriptional activity based on analysis of intron coverage from total RNA-seq data. Comparison of the results derived from iRNA-seq analyses with parallel results derived using current methods for genome-wide determination of transcriptional activity, i.e. global run-on (GRO)-seq and RNA polymerase II (RNAPII) ChIP-seq, demonstrate that iRNA-seq provides similar results in terms

SUBMITTER: Madsen JG 

PROVIDER: S-EPMC4381047 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature