Improvement of IFNg ELISPOT Performance Following Overnight Resting of Frozen PBMC Samples Confirmed Through Rigorous Statistical Analysis.
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ABSTRACT:
SUBMITTER: Santos R
PROVIDER: S-EPMC4381205 | biostudies-literature | 2014 Dec
REPOSITORIES: biostudies-literature
Publications
Improvement of IFNg ELISPOT Performance Following Overnight Resting of Frozen PBMC Samples Confirmed Through Rigorous Statistical Analysis.
Santos Radleigh R Buying Alcinette A Sabri Nazila N Yu John J Gringeri Anthony A Bender James J Janetzki Sylvia S Pinilla Clemencia C Judkowski Valeria A VA
Cells 20141224 1
Immune monitoring of functional responses is a fundamental parameter to establish correlates of protection in clinical trials evaluating vaccines and therapies to boost antigen-specific responses. The IFNg ELISPOT assay is a well-standardized and validated method for the determination of functional IFNg-producing T-cells in peripheral blood mononuclear cells (PBMC); however, its performance greatly depends on the quality and integrity of the cryopreserved PBMC. Here, we investigate the effect of ...[more]
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