The pluripotent regulatory circuitry connecting promoters to their long-range interacting elements.

ABSTRACT: The mammalian genome harbors up to one million regulatory elements often located at great distances from their target genes. Long-range elements control genes through physical contact with promoters and can be recognized by the presence of specific histone modifications and transcription factor binding. Linking regulatory elements to specific promoters genome-wide is currently impeded by the limited resolution of high-throughput chromatin interaction assays. Here we apply a sequence capture approach to enrich Hi-C libraries for >22,000 annotated mouse promoters to identify statistically significant, long-range interactions at restriction fragment resolution, assigning long-range interacting elements to their target genes genome-wide in embryonic stem cells and fetal liver cells. The distal

SUBMITTER: Schoenfelder S 

PROVIDER: S-EPMC4381529 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature