Unknown

Dataset Information

0

A cost-effective RNA sequencing protocol for large-scale gene expression studies.

ABSTRACT: RNA sequencing has increasingly become an indispensable tool for biological research. While sequencing costs have fallen dramatically in recent years, the current cost of RNA sequencing, nonetheless, remains a barrier to even more widespread adoption. Here, we present a simple RNA sequencing protocol with substantially reduced costs. This protocol uses as little as 10?ng of total RNA, allows multiplex sequencing of up to 96 samples per lane, and is strand specific. Extensive validation using human embryonic stem cells showed high consistency between technical replicates at various multiplexing levels.

SUBMITTER: Hou Z 

PROVIDER: S-EPMC4381617 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

A cost-effective RNA sequencing protocol for large-scale gene expression studies.

Hou Zhonggang Z   Jiang Peng P   Swanson Scott A SA   Elwell Angela L AL   Nguyen Bao Kim S BK   Bolin Jennifer M JM   Stewart Ron R   Thomson James A JA  

Scientific reports 20150401

RNA sequencing has increasingly become an indispensable tool for biological research. While sequencing costs have fallen dramatically in recent years, the current cost of RNA sequencing, nonetheless, remains a barrier to even more widespread adoption. Here, we present a simple RNA sequencing protocol with substantially reduced costs. This protocol uses as little as 10 ng of total RNA, allows multiplex sequencing of up to 96 samples per lane, and is strand specific. Extensive validation using hum  ...[more]

Similar Datasets

Transcriptomics A cost-effective RNA sequencing protocol for large-scale gene expression studies
2016-11-15 | GSE89872 | GEO
Transcriptomics Large-scale low-cost NGS library preparation using a robust Tn5 purification and tagmentation protocol
2017-11-13 | GSE101520 | GEO
Transcriptomics A simple, cost-effective, and robust method for rRNA depletion in RNA-sequencing studies
2019-12-28 | GSE142656 | GEO
Unknown A Simple, Cost-Effective, and Robust Method for rRNA Depletion in RNA-Sequencing Studies.
| S-EPMC7175087 | biostudies-literature
Unknown Gene expression variability and the analysis of large-scale RNA-seq studies with the MDSeq.
| S-EPMC5737414 | biostudies-literature
Unknown RNA pre-amplification enables large-scale RT-qPCR gene-expression studies on limiting sample amounts.
| S-EPMC2789097 | biostudies-literature
Unknown Efficient gene-environment interaction tests for large biobank-scale sequencing studies.
| S-EPMC7754763 | biostudies-literature
Transcriptomics Large-scale low-cost NGS library preparation using a robust Tn5 purification and tagmentation protocol [i5i7]
2017-11-13 | GSE101518 | GEO
Transcriptomics Large-scale low-cost NGS library preparation using a robust Tn5 purification and tagmentation protocol [polyA]
2017-11-13 | GSE101519 | GEO
Unknown MiRonTop: mining microRNAs targets across large scale gene expression studies.
| S-EPMC2995122 | biostudies-literature