Plasmodium knowlesi genome sequences from clinical isolates reveal extensive genomic dimorphism.

ABSTRACT: Plasmodium knowlesi is a newly described zoonosis that causes malaria in the human population that can be severe and fatal. The study of P. knowlesi parasites from human clinical isolates is relatively new and, in order to obtain maximum information from patient sample collections, we explored the possibility of generating P. knowlesi genome sequences from archived clinical isolates. Our patient sample collection consisted of frozen whole blood samples that contained excessive human DNA contamination and, in that form, were not suitable for parasite genome sequencing. We developed a method to reduce the amount of human DNA in the thawed blood samples in preparation for high throughput parasite genome sequencing using Illumina HiSeq and MiSeq sequencing platforms. Seven of fifteen samples p

SUBMITTER: Pinheiro MM 

PROVIDER: S-EPMC4382175 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature