Application of multiplex arrays for cytokine and chemokine profiling of bile.

ABSTRACT:

Background

Gallbladder disease is highly related to inflammation, but the inflammatory processes are not well understood. Bile provides a direct substrate in assessing the local inflammatory response that develops in the gallbladder. To assess the reproducibility of measuring inflammatory markers in bile, we designed a methods study of 69 multiplexed immune-related markers measured in bile obtained from gallstone patients.

Methods

To evaluate assay performance, a total of 18 bile samples were tested twice within the same plate for each analyte, and the 18 bile samples were tested on two different days for each analyte. We used the following performance parameters: detectability, coefficient of variation (CV), intraclass correlation coefficient (ICC), and percent agreement (c

SUBMITTER: Kemp TJ 

PROVIDER: S-EPMC4382212 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature