Selection of reference genes for transcriptional analysis of edible tubers of potato (Solanum tuberosum L.).

ABSTRACT: Potato (Solanum tuberosum) yield has increased dramatically over the last 50 years and this has been achieved by a combination of improved agronomy and biotechnology efforts. Gene studies are taking place to improve new qualities and develop new cultivars. Reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) is a bench-marking analytical tool for gene expression analysis, but its accuracy is highly dependent on a reliable normalization strategy of an invariant reference genes. For this reason, the goal of this work was to select and validate reference genes for transcriptional analysis of edible tubers of potato. To do so, RT-qPCR primers were designed for ten genes with relatively stable expression in potato tubers as observed in RNA-Seq experiments. Primers were designe

SUBMITTER: Mariot RF 

PROVIDER: S-EPMC4382326 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature