High quality RNA in semen and sperm: isolation, analysis and potential application in clinical testing.

ABSTRACT:

Purpose

Male infertility is a complex health condition. To our knowledge there are no molecular biomarkers of male infertility. Sperm RNA is a potential biomarker for detecting sperm abnormalities and viability at infertility clinics. However, RNA use is hindered by its inconsistent quantity, quality, multiple cell types in semen and condensed sperm structure.

Materials and methods

We tested the usefulness of high quality RNA isolated from mature sperm and whole semen by our protocol, which reduces RNA degradation by maintaining semen and protocol components at 37 C and decreasing processing time. We isolated RNA from 83 whole semen samples, 18 samples of motile sperm prepared by the swim-up protocol and 18 of sperm prepared by the standard Percoll gradient method.

Result

SUBMITTER: Georgiadis AP 

PROVIDER: S-EPMC4382362 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature