Image fusion of mass spectrometry and microscopy: a multimodality paradigm for molecular tissue mapping.

ABSTRACT: We describe a predictive imaging modality created by 'fusing' two distinct technologies: imaging mass spectrometry (IMS) and microscopy. IMS-generated molecular maps, rich in chemical information but having coarse spatial resolution, are combined with optical microscopy maps, which have relatively low chemical specificity but high spatial information. The resulting images combine the advantages of both technologies, enabling prediction of a molecular distribution both at high spatial resolution and with high chemical specificity. Multivariate regression is used to model variables in one technology, using variables from the other technology. We demonstrate the potential of image fusion through several applications: (i) 'sharpening' of IMS images, which uses microscopy measurements to predic

SUBMITTER: Van de Plas R 

PROVIDER: S-EPMC4382398 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature