Frequent CALR exon 9 alterations in JAK2 V617F-mutated essential thrombocythemia detected by high-resolution melting analysis.
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ABSTRACT:
SUBMITTER: Lim KH
PROVIDER: S-EPMC4382662 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Publications
Frequent CALR exon 9 alterations in JAK2 V617F-mutated essential thrombocythemia detected by high-resolution melting analysis.
Lim K-H KH Chang Y-C YC Gon-Shen Chen C C Lin H-C HC Wang W-T WT Chiang Y-H YH Cheng H-I HI Su N-W NW Lin J J Chang Y-F YF Chang M-C MC Hsieh R-K RK Kuo Y-Y YY Chou W-C WC
Blood cancer journal 20150320
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Project description:The aim of the study was to evaluate selected angiogenic factors in patients with essential thrombocythemia (ET) depending on JAK2V617F, calreticulin gene (CALR) and myeloproliferative leukemia virus oncogene (MPL) mutations. Sixty ET patients and 20 healthy volunteers were enrolled in the study. The following tests were performed: vascular endothelial growth factor- A (VEGF-A), soluble vascular endothelial growth factor receptor-1 (sVEGFR-1),soluble vascular endothelial growth factor receptor-2 (sVEGFR-2), platelet-derived growth factor( PDGF-BB), and stromal-derived factor-1α (SDF-1α). We observed an increased PDGF-BB level in patients with ET compared to the controls. Patients with CALR mutation had significantly higher concentration of PDGF-BB and lower concentration of SDF-1α than patients with JAK2V617F mutation. High concentration of PDGF-BB and low concentration of SDF-1α in patients with CALR(+) ET may indicate a contribution of these chemokines in disturbed Ca2+ metabolism in platelets.
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