CRISPRdirect: software for designing CRISPR/Cas guide RNA with reduced off-target sites.

ABSTRACT:

Unlabelled

CRISPRdirect is a simple and functional web server for selecting rational CRISPR/Cas targets from an input sequence. The CRISPR/Cas system is a promising technique for genome engineering which allows target-specific cleavage of genomic DNA guided by Cas9 nuclease in complex with a guide RNA (gRNA), that complementarily binds to a ∼ 20 nt targeted sequence. The target sequence requirements are twofold. First, the 5'-NGG protospacer adjacent motif (PAM) sequence must be located adjacent to the target sequence. Second, the target sequence should be specific within the entire genome in order to avoid off-target editing. CRISPRdirect enables users to easily select rational target sequences with minimized off-target sites by performing exhaustive searches against genomic seque

SUBMITTER: Naito Y 

PROVIDER: S-EPMC4382898 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature