Targeting class I histone deacetylase 2 in MYC amplified group 3 medulloblastoma.

ABSTRACT:

Introduction

Medulloblastoma (MB) is the most frequent malignant brain tumor in children. Four subgroups with distinct genetic, epigenetic and clinical characteristics have been identified. Survival remains particularly poor in patients with Group 3 tumors harbouring a MYC amplification. We herein explore the molecular mechanisms and translational implications of class I histone deacetylase (HDAC) inhibition in MYC driven MBs.

Material and methods

Expression of HDACs in primary MB subgroups was compared to normal brain tissue. A panel of MB cell lines, including Group 3 MYC amplified cell lines, were used as model systems. Cells were treated with HDAC inhibitors (HDACi) selectively targeting class I or IIa HDACs. Depletion of HDAC2 was performed. Intracellular HDAC activity,

SUBMITTER: Ecker J 

PROVIDER: S-EPMC4382927 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature