Dissecting meiotic recombination based on tetrad analysis by single-microspore sequencing in maize.

ABSTRACT: Meiotic recombination drives eukaryotic sexual reproduction and the generation of genome diversity. Tetrad analysis, which examines the four chromatids resulting from a single meiosis, is an ideal method to study the mechanisms of homologous recombination. Here we develop a method to isolate the four microspores from a single tetrad in maize for the purpose of whole-genome sequencing. A high-resolution recombination map reveals that crossovers are unevenly distributed across the genome and are more likely to occur in the genic than intergenic regions, especially common in the 5'- and 3'-end regions of annotated genes. The direct detection of genomic exchanges suggests that conversions likely occur in most crossover tracts. Negative crossover interference and weak chromatid interference are

SUBMITTER: Li X 

PROVIDER: S-EPMC4383000 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature