Major protein alterations in spermatozoa from infertile men with unilateral varicocele.

ABSTRACT:

Background

The etiology of varicocele, a common cause of male factor infertility, remains unclear. Proteomic changes responsible for the underlying pathology of unilateral varicocele have not been evaluated. The objective of this prospective study was to employ proteomic techniques and bioinformatic tools to identify and analyze proteins of interest in infertile men with unilateral varicocele.

Methods

Spermatozoa from infertile men with unilateral varicocele (n=5) and from fertile men (control; n=5) were pooled in two groups respectively. Proteins were extracted and separated by 1-D SDS-PAGE. Bands were digested and identified on a LTQ-Orbitrap Elite hybrid mass spectrometer system. Bioinformatic analysis identified the pathways and functions of the differentially expressed

SUBMITTER: Agarwal A 

PROVIDER: S-EPMC4383193 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature