Pancreatic Cancer-Derived Exosomes Cause Paraneoplastic β-cell Dysfunction.

ABSTRACT:

Purpose

Pancreatic cancer frequently causes diabetes. We recently proposed adrenomedullin as a candidate mediator of pancreatic β-cell dysfunction in pancreatic cancer. How pancreatic cancer-derived adrenomedullin reaches β cells remote from the cancer to induce β-cell dysfunction is unknown. We tested a novel hypothesis that pancreatic cancer sheds adrenomedullin-containing exosomes into circulation, which are transported to β cells and impair insulin secretion.

Experimental methods

We characterized exosomes from conditioned media of pancreatic cancer cell lines (n = 5) and portal/peripheral venous blood of patients with pancreatic cancer (n = 20). Western blot analysis showed the presence of adrenomedullin in pancreatic cancer-exosomes. We determined the effect of adrenome

SUBMITTER: Javeed N 

PROVIDER: S-EPMC4383684 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature