Image cytometry-based detection of aneuploidy by fluorescence in situ hybridization in suspension.

ABSTRACT: Cytogenetic abnormalities are important diagnostic and prognostic criteria for hematologic malignancies. Karyotyping and fluorescence in situ hybridization (FISH) are the conventional methods by which these abnormalities are detected. The sensitivity of these microscopy-based methods is limited by the abundance of the abnormal cells in the samples and therefore these analyses are commonly not applicable to minimal residual disease (MRD) stages. A flow cytometry-based imaging approach was developed to detect chromosomal abnormalities following FISH in suspension (FISH-IS), which enables the automated analysis of several log-magnitude higher number of cells compared with the microscopy-based approaches. This study demonstrates the applicability of FISH-IS for detecting numerical chromosome a

SUBMITTER: Minderman H 

PROVIDER: S-EPMC4384178 | biostudies-literature | 2012 Sep

REPOSITORIES: biostudies-literature