MicroRNA-29c mediates initiation of gastric carcinogenesis by directly targeting ITGB1.

ABSTRACT:

Objective

Gastric cancer (GC) remains difficult to cure due to heterogeneity in a clinical challenge and the molecular mechanisms underlying this disease are complex and not completely understood. Accumulating evidence suggests that microRNAs (miRNAs) play an important role in GC, but the role of specific miRNAs involved in this disease remains elusive. We performed next generation sequencing (NGS)-based whole-transcriptome profiling to discover GC-specific miRNAs, followed by functional validation of results.

Design

NGS-based miRNA profiles were generated in matched pairs of GCs and adjacent normal mucosa (NM). Quantitative RT-PCR validation of miR-29c expression was performed in 274 gastric tissues, which included two cohorts of matched GC and NM specimens. Functional vali

SUBMITTER: Han TS 

PROVIDER: S-EPMC4384419 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature