RhoB regulates PDGFR-beta trafficking and signaling in vascular smooth muscle cells.

ABSTRACT:

Objective

RhoB is a small GTPase localized at the plasma membrane and endosomes that participates in the regulation of endocytic trafficking of the epidermal growth factor (EGF) receptor and the nonreceptor kinases Src and Akt. This study was performed to determine whether RhoB plays a critical role in trafficking and signaling by the platelet-derived growth factor receptor-beta (PDGFR-beta) in vascular smooth muscle cells.

Methods and results

Cells derived from RhoB knockout mice failed to proliferate in response to PDGF, and downstream signaling was compromised as reflected by reduced phosphorylation of the effector kinases Akt and ERK1/2. In normal cells, PDGF stimulated trafficking of PDGFR-beta into a perinuclear late endosomal compartment and triggered entry of Src, Ak

SUBMITTER: Huang M 

PROVIDER: S-EPMC4384698 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature