Characterization of a lipase from a newly isolated Pseudomonas sp.

ABSTRACT:

Background and objectives

Lipases are valuable biocatalysts which are widely used in the detergent, food, dairy and pharmaceutical industries. The aims of the present study included the isolation of a lipase-producer from industrial zones and the partial characterization of the enzyme.

Materials and methods

A number of bacteria were isolated from sites related to the oil industries. An isolate forming a halo zone in a selective medium (TW agar) was then selected and grown on a medium suitable for the production of lipase. The isolate was subsequently identified by the 16S rRNA sequencing method, and its enzyme activity was measured by a spectrophotometer using pNPP as a substrate.

Results

The selected isolate was identified by the molecular method as Pseudomonas sp. I

SUBMITTER: Noormohamadi R 

PROVIDER: S-EPMC4385172 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature