Sponge-mediated lentivirus delivery to acute and chronic spinal cord injuries.

ABSTRACT: The environment within the spinal cord after injury, which changes in the progression from the acute to chronic stages, limits the extent of regeneration. The delivery of inductive factors to promote regeneration following spinal cord injury has been promising, yet, few strategies are versatile to allow delivery during acute or chronic injury that would facilitate screening of candidate therapies. This report investigates the intrathecal delivery of lentiviruses for long-term expression of regenerative factors. Lentivirus-filled sponges were inserted into the intrathecal space surrounding the spinal cord, with transgene expression observed within multiple cell types that persists for 12 weeks for both intact and injured spinal cord, without any apparent damage to the spinal cord tissue. Sp

SUBMITTER: Thomas AM 

PROVIDER: S-EPMC4385414 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature