Characterization of metabolites determined by means of 1H HR MAS NMR in intervertebral disc degeneration.

ABSTRACT:

Object

The objective of this study is the identification of metabolites by means of (1)H high resolution magic angle spinning nuclear magnetic resonance ((1)H HR MAS NMR) spectroscopy and the evaluation of their applicability in distinguishing between healthy and degenerated disc tissues.

Materials and methods

Differences between the metabolic profiles of healthy and degenerated disc tissues were studied by means of (1)H HR MAS NMR. Analysis was performed for 81 disc tissue samples (control samples n = 21, degenerated disc tissue samples n = 60). Twenty six metabolites (amino acids, carbohydrates, and alcohols) were identified and quantified.

Results

The results indicate that the metabolic profile of degenerated discs is characterized by the presence of 2-propanol and

SUBMITTER: Pacholczyk-Sienicka B 

PROVIDER: S-EPMC4385564 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature