Combinatorial proteomic analysis of intercellular signaling applied to the CD28 T-cell costimulatory receptor.

ABSTRACT: Systematic characterization of intercellular signaling approximating the physiological conditions of stimulation that involve direct cell-cell contact is challenging. We describe a proteomic strategy to analyze physiological signaling mediated by the T-cell costimulatory receptor CD28. We identified signaling pathways activated by CD28 during direct cell-cell contact by global analysis of protein phosphorylation. To define immediate CD28 targets, we used phosphorylated forms of the CD28 cytoplasmic region to obtain the CD28 interactome. The interaction profiles of selected CD28-interacting proteins were further characterized in vivo for amplifying the CD28 interactome. The combination of the global phosphorylation and interactome analyses revealed broad regulation of CD28 and its interacto

SUBMITTER: Tian R 

PROVIDER: S-EPMC4386406 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature