Ex vivo four-dimensional lung cancer model mimics metastasis.

ABSTRACT:

Background

We have developed a four-dimensional (4D) lung cancer model that forms perfusable tumor nodules. We determined if the model could be modified to mimic metastasis.

Methods

We modified the 4D lung cancer model by seeding H1299, A549, or H460 cells through the trachea only to the left lobes of the acellular lung matrix. The model was modified so that the tumor cells can reach the right lobes of the acellular lung matrix only through the pulmonary artery as circulating tumor cells (CTC). We determined the gene expressions of the primary tumor, CTCs, and metastatic lesions using the Human OneArray chip.

Results

All cell lines formed a primary tumor in the left lobe of the ex vivo 4D lung cancer model. The CTCs were identified in the media and increased over time

SUBMITTER: Mishra DK 

PROVIDER: S-EPMC4387006 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature