Food antigen-induced immune responses in Crohn's disease patients and experimental colitis mice.

ABSTRACT:

Background

In Crohn's disease (CD), the involvement of food antigens in immune responses remains unclear. The objective of this study was to detect immune responses against food antigens in CD patients and examine the mechanism in a mouse model of colitis.

Methods

We enrolled 98 CD patients, 50 ulcerative colitis patients, and 52 healthy controls (HCs) to compare the levels of serum immunoglobulin (Ig)Gs against 88 foods. The presence of serum IgGs against foods was also examined in interleukin (IL)-10 knockout (KO) mice in which CD4(+) T cell activation by antigenic food protein was assessed. Mice transferred with IL-10 KO cells received diets with or without food antigens, and the development of colitis was evaluated.

Results

The prevalence of IgGs against various f

SUBMITTER: Kawaguchi T 

PROVIDER: S-EPMC4387251 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature