Sources of variability in cytosolic calcium transients triggered by stimulation of homogeneous uro-epithelial cell monolayers.

ABSTRACT: Epithelial tissue structure is the emergent outcome of the interactions between large numbers of individual cells. Experimental cell biology offers an important tool to unravel these complex interactions, but current methods of analysis tend to be limited to mean field approaches or representation by selected subsets of cells. This may result in bias towards cells that respond in a particular way and/or neglect local, context-specific cell responses. Here, an automated algorithm was applied to examine in detail the individual calcium transients evoked in genetically homogeneous, but asynchronous populations of cultured non-immortalized normal human urothelial cells when subjected to either the global application of an external agonist or a localized scratch wound. The recorded calcium tran

SUBMITTER: Appleby PA 

PROVIDER: S-EPMC4387530 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature