Membrane adsorbers comprising grafted glycopolymers for targeted lectin binding.

ABSTRACT: This work details the design and testing of affinity membrane adsorbers for lectin purifications that incorporate glucose-containing glycopolymers. It is the selective interaction between the sugar residues of the glycopolymer and the complementary carbohydrate-binding domain of the lectin that provides the basis for the isolation and purification of lectins from complex biological media. The design approach used in these studies was to graft glycopolymer 'tentacles' from macroporous regenerated cellulose membranes by atom transfer radical polymerization. As shown in earlier studies, this design approach can be used to prepare high-productivity membrane adsorbers. The model lectin, concanavalin A (conA), was used to evaluate membrane performance in bind-and-elute purification, using a low

SUBMITTER: Chenette HC 

PROVIDER: S-EPMC4387574 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature