In vitro exposure to very low-level laser modifies expression level of extracellular matrix protein RNAs and mitochondria dynamics in mouse embryonic fibroblasts.

ABSTRACT:

Background

Low-level lasers working at 633 or 670 nm and emitting extremely low power densities (Ultra Low Level Lasers - ULLL) exert an overall effect of photobiostimulation on cellular metabolism and energy balance. In previous studies, it was demonstrated that ULLL pulsed emission mode regulates neurite elongation in vitro and exerts protective action against oxidative stress.

Methods

In this study the action of ULLL supplied in both pulsed and continuous mode vs continuous LLL on fibroblast cultures (Mouse Embryonic Fibroblast-MEF) was tested, focusing on mitochondria network and the expression level of mRNA encoding for proteins involved in the cell-matrix adhesion.

Results

It was shown that ULLL at 670 nm, at extremely low average power output (0.21 mW/ cm(2)) a

SUBMITTER: Giuliani A 

PROVIDER: S-EPMC4387590 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature