A novel, rapid method to quantify intraplatelet calcium dynamics by ratiometric flow cytometry.

ABSTRACT: Cytosolic free calcium ions represent important second-messengers in platelets. Therefore, quantitative measurement of intraplatelet calcium provides a popular and very sensitive tool to evaluate platelet activation and reactivity. Current protocols for determination of intracellular calcium concentrations in platelets have a number of limitations. Cuvette-based methods do not allow measurement of calcium flux in complex systems, such as whole blood, and therefore require isolation steps that potentially interfere with platelet activation. Flow cytometry has the potential to overcome this limitation, but to date the application of calibrated, quantitative readout of calcium kinetics has only been described for Indo-1. As excitation of Indo-1 requires a laser in the ultraviolet range, such

SUBMITTER: Assinger A 

PROVIDER: S-EPMC4388375 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature