A multistep high-content screening approach to identify novel functionally relevant target genes in pancreatic cancer.

ABSTRACT: In order to foster the systematic identification of novel genes with important functional roles in pancreatic cancer, we have devised a multi-stage screening strategy to provide a rational basis for the selection of highly relevant novel candidate genes based on the results of functional high-content analyses. The workflow comprised three consecutive stages: 1) serial gene expression profiling analyses of primary human pancreatic tissues as well as a number of in vivo and in vitro models of tumor-relevant characteristics in order to identify genes with conspicuous expression patterns; 2) use of 'reverse transfection array' technology for large-scale parallelized functional analyses of potential candidate genes in cell-based assays; and 3) selection of individual candidate genes for further

SUBMITTER: Buchholz M 

PROVIDER: S-EPMC4388713 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature