Loss of sirtuin 1 (SIRT1) disrupts skin barrier integrity and sensitizes mice to epicutaneous allergen challenge.

ABSTRACT:

Background

Skin barrier integrity requires a highly coordinated molecular system involving the structural protein filaggrin (FLG). Mutational loss of the skin barrier protein FLG predisposes subjects to the development of atopic dermatitis (AD).

Objective

We sought to determine the role of sirtuin 1 (SIRT1) in skin barrier function, FLG expression, and development of AD.

Methods

Skin histology of mice with skin-specific SIRT1 deletion and wild-type control animals was examined by using hematoxylin and eosin staining. Protein and mRNA abundance was analyzed by means of immunoblotting, immunohistochemistry, immunofluorescence, and RT-PCR. Serum antibody levels were assessed by means of ELISA.

Results

Here we show that FLG is regulated by the protein deacetylase S

SUBMITTER: Ming M 

PROVIDER: S-EPMC4388755 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature