Effect of different heterotrophic plate count methods on the estimation of the composition of the culturable microbial community.

ABSTRACT: Heterotrophic plate counts (HPC) are routinely determined within the scope of water quality assessment. However, variable HPC methods with different cultivation parameters (i.e., temperature and media type) are applied, which could lead to significant effects in the outcome of the analysis. Therefore the effect of different HPC methods, according to DIN EN ISO 6222 and EPA, on the culturable microbial community composition was investigated by 16S rRNA gene sequence analysis and statistical evaluation was performed. The culturable community composition revealed significant effects assigned to temperature (p < 0.01), while for media type no statistical significance was observed. However, the abundance of certain detected bacteria was affected. Lower temperature (22 °C) showed the abundance o

SUBMITTER: Gensberger ET 

PROVIDER: S-EPMC4389272 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature