The tumour-promoting receptor tyrosine kinase, EphB4, regulates expression of integrin-β8 in prostate cancer cells.

ABSTRACT:

Background

The EphB4 receptor tyrosine kinase is overexpressed in many cancers including prostate cancer. The molecular mechanisms by which this ephrin receptor influences cancer progression are complex as there are tumor-promoting ligand-independent mechanisms in place as well as ligand-dependent tumor suppressive pathways.

Methods

We employed transient knockdown of EPHB4 in prostate cancer cells, coupled with gene microarray analysis, to identify genes that were regulated by EPHB4 and may represent linked tumor-promoting factors. We validated target genes using qRT-PCR and employed functional assays to determine their role in prostate cancer migration and invasion.

Results

We discovered that over 500 genes were deregulated upon EPHB4 siRNA knockdown, with integrin β

SUBMITTER: Mertens-Walker I 

PROVIDER: S-EPMC4389669 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature