The role of multipotent cancer associated fibroblasts in hepatocarcinogenesis.

ABSTRACT:

Background

The presence of tumor supporting cells in various cancer, including in hepatocellular carcinoma (HCC), has become an important target in the study of carcinogenesis. The cancer-associated fibroblast (CAF), one of the most important cellular components in the cancer stroma, might contribute to the progression of the disease due to its plasticity, a behavior of the stem cells. In this study, we investigate the significance of the CAF and its role in the HCC progression and metastasis.

Methods

Primary CAF and non-tumoral fibroblast (NTF) from nine paired HCC and distant non-tumoral liver tissues were isolated and cultured. The cells were characterized by flow cytometry, RT-PCR, anchorage-independent assay and in vitro cells directed trans-differentiation. Co-culture

SUBMITTER: Sukowati CH 

PROVIDER: S-EPMC4389787 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature