A temperature-sensitive expression system based on the Geobacillus stearothermophilus NRS 2004/3a sgsE surface-layer gene promoter.

ABSTRACT: The sgsE gene coding for the S-layer (surface layer) protein in the thermophilic Gram-positive bacterium Geobacillus stearothermophilus NRS 2004/3a is strongly induced when the culture is shifted from optimal (55 degrees C) to maximally tolerable growth temperature (67 degrees C). Here, we investigated the regulation of the sgsE promoter in G. stearothermophilus and tested the function of this promoter in Bacillus subtilis. We used EGFP (enhanced green fluorescent protein) reporter constructs and found that the sgsE promoter has very low basal activity at 28 degrees C, but is approx. 20-fold induced by elevated growth temperatures (37 and 45 degrees C). The promoter confers high expression levels, as EGFP mRNA levels at 45 degrees C were approx. 120-fold more abundant than mRNA levels of t

SUBMITTER: Novotny R 

PROVIDER: S-EPMC4389859 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature