Trophectoderm DNA fingerprinting by quantitative real-time PCR successfully distinguishes sibling human embryos.

ABSTRACT:

Purpose

To validate a novel and more practical system for trophectoderm DNA fingerprinting which reliably distinguishes sibling embryos from each other.

Methods

In this prospective and blinded study two-cell and 5-cell samples from commercially available sibling cell lines and excess DNA from trophectoderm biopsies of sibling human blastocysts were evaluated for accurate assignment of relationship using qPCR-based allelic discrimination from 40 single nucleotide polymorphisms (SNPs) with low allele frequency variation and high heterozygosity.

Results

Cell samples with self relationships averaged 95.1 ± 5.9 % similarity. Sibling relationships averaged 57.2 ± 5.9 % similarity for all 40 SNPs, and 40.8 ± 8.2 % similarity for the 25 informative SNPs. Assignment of relatio

SUBMITTER: Scott RT 

PROVIDER: S-EPMC4389931 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature