Genotyping common FSHR polymorphisms based on competitive amplification of differentially melting amplicons (CADMA).

ABSTRACT:

Purpose

To provide an improved platform for simple, reliable, and cost-effective genotyping.

Background

Modern fertility treatments are becoming increasingly individualized in an attempt to optimise the follicular response and reproductive outcome, following controlled ovarian stimulation. As the field of pharmacogenetics evolve, genetic biomarkers such as polymorphisms of the follicle stimulating hormone receptor (FSHR) may be included as a predictive tool for individualized fertility treatment. However, the currently available genotyping methods are expensive, time-consuming or have a limited analytical sensitivity. Here, we present a novel version of "competitive amplification of differentially melting amplicons" (CADMA), providing an improved platform for simple, reliabl

SUBMITTER: Borgbo T 

PROVIDER: S-EPMC4389944 | biostudies-literature | 2014 Nov

REPOSITORIES: biostudies-literature