Rapid mass spectrometric conversion of tissue biopsy samples into permanent quantitative digital proteome maps.

ABSTRACT: Clinical specimens are each inherently unique, limited and nonrenewable. Small samples such as tissue biopsies are often completely consumed after a limited number of analyses. Here we present a method that enables fast and reproducible conversion of a small amount of tissue (approximating the quantity obtained by a biopsy) into a single, permanent digital file representing the mass spectrometry (MS)-measurable proteome of the sample. The method combines pressure cycling technology (PCT) and sequential window acquisition of all theoretical fragment ion spectra (SWATH)-MS. The resulting proteome maps can be analyzed, re-analyzed, compared and mined in silico to detect and quantify specific proteins across multiple samples. We used this method to process and convert 18 biopsy samples from ni

SUBMITTER: Guo T 

PROVIDER: S-EPMC4390165 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature