Transgene expression and Bt protein content in transgenic Bt maize (MON810) under optimal and stressful environmental conditions.

ABSTRACT: Bt protein content in transgenic insect resistant (Bt) maize may vary between tissues within plants and between plants growing under different environmental conditions. However, it is unknown whether and how Bt protein content correlates with transgene expression, and whether this relationship is influenced by stressful environmental conditions. Two Bt maize varieties containing the same transgene cassette (MON 810) were grown under optimal and stressful conditions. Before and during stress exposure, the upper leaves were analysed for transgene expression using quantitative RT-PCR and for Bt content using ELISA. Under optimal conditions there was no significant difference in the transgene expression between the two investigated Bt maize varieties whereas Bt protein content differed signifi

SUBMITTER: Trtikova M 

PROVIDER: S-EPMC4390241 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature