Quantification of extracellular matrix proteins from a rat lung scaffold to provide a molecular readout for tissue engineering.

ABSTRACT: The use of extracellular matrix (ECM) scaffolds, derived from decellularized tissues for engineered organ generation, holds enormous potential in the field of regenerative medicine. To support organ engineering efforts, we developed a targeted proteomics method to extract and quantify extracellular matrix components from tissues. Our method provides more complete and accurate protein characterization than traditional approaches. This is accomplished through the analysis of both the chaotrope-soluble and -insoluble protein fractions and using recombinantly generated stable isotope labeled peptides for endogenous protein quantification. Using this approach, we have generated 74 peptides, representing 56 proteins to quantify protein in native (nondecellularized) and decellularized lung matric

SUBMITTER: Hill RC 

PROVIDER: S-EPMC4390273 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature