Unravelling glucan recognition systems by glycome microarrays using the designer approach and mass spectrometry.

ABSTRACT: Glucans are polymers of d-glucose with differing linkages in linear or branched sequences. They are constituents of microbial and plant cell-walls and involved in important bio-recognition processes, including immunomodulation, anticancer activities, pathogen virulence, and plant cell-wall biodegradation. Translational possibilities for these activities in medicine and biotechnology are considerable. High-throughput micro-methods are needed to screen proteins for recognition of specific glucan sequences as a lead to structure-function studies and their exploitation. We describe construction of a "glucome" microarray, the first sequence-defined glycome-scale microarray, using a "designer" approach from targeted ligand-bearing glucans in conjunction with a novel high-sensitivity mass spectro

SUBMITTER: Palma AS 

PROVIDER: S-EPMC4390274 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature