Target-dependent enrichment of virions determines the reduction of high-throughput sequencing in virus discovery.

ABSTRACT: Viral infections cause many different diseases stemming both from well-characterized viral pathogens but also from emerging viruses, and the search for novel viruses continues to be of great importance. High-throughput sequencing is an important technology for this purpose. However, viral nucleic acids often constitute a minute proportion of the total genetic material in a sample from infected tissue. Techniques to enrich viral targets in high-throughput sequencing have been reported, but the sensitivity of such methods is not well established. This study compares different library preparation techniques targeting both DNA and RNA with and without virion enrichment. By optimizing the selection of intact virus particles, both by physical and enzymatic approaches, we assessed the effectivene

SUBMITTER: Jensen RH 

PROVIDER: S-EPMC4390369 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature