Gene regulation by H-NS as a function of growth conditions depends on chromosomal position in Escherichia coli.

ABSTRACT: Cellular adaptation to changing environmental conditions requires the coordinated regulation of expression of large sets of genes by global regulatory factors such as nucleoid associated proteins. Although in eukaryotic cells genomic position is known to play an important role in regulation of gene expression, it remains to be established whether in bacterial cells there is an influence of chromosomal position on the efficiency of these global regulators. Here we show for the first time that genome position can affect transcription activity of a promoter regulated by the histone-like nucleoid-structuring protein (H-NS), a global regulator of bacterial transcription and genome organization. We have used as a local reporter of H-NS activity the level of expression of a fluorescent reporter p

SUBMITTER: Brambilla E 

PROVIDER: S-EPMC4390576 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature