Multiplex Real-Time PCR Method for Simultaneous Identification and Toxigenic Type Characterization of Clostridium difficile From Stool Samples.

ABSTRACT:

Background

The aim of this study was to develop and validate a multiplex real-time PCR assay for simultaneous identification and toxigenic type characterization of Clostridium difficile.

Methods

The multiplex real-time PCR assay targeted and simultaneously detected triose phosphate isomerase (tpi) and binary toxin (cdtA) genes, and toxin A (tcdA) and B (tcdB) genes in the first and sec tubes, respectively. The results of multiplex real-time PCR were compared to those of the BD GeneOhm Cdiff assay, targeting the tcdB gene alone. The toxigenic culture was used as the reference, where toxin genes were detected by multiplex real-time PCR.

Results

A total of 351 stool samples from consecutive patients were included in the study. Fifty-five stool samples (15.6%) were determ

SUBMITTER: Kilic A 

PROVIDER: S-EPMC4390698 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature