13C, 2h NMR studies of structural and dynamical modifications of glucose-exposed porcine aortic elastin.

ABSTRACT: Elastin, the principal component of the elastic fiber of the extracellular matrix, imparts to vertebrate tissues remarkable resilience and longevity. This work focuses on elucidating dynamical and structural modifications of porcine aortic elastin exposed to glucose by solid-state NMR spectroscopic and relaxation methodologies. Results from macroscopic stress-strain tests are also presented and indicate that glucose-treated elastin is mechanically stiffer than the same tissue without glucose treatment. These measurements show a large hysteresis in the stress-strain behavior of glucose-treated elastin-a well-known signature of viscoelasticity. Two-dimensional relaxation NMR methods were used to investigate the correlation time, distribution, and population of water in these samples. Differe

SUBMITTER: Silverstein MC 

PROVIDER: S-EPMC4390820 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature