Experimental verification of the kinetic theory of FRET using optical microspectroscopy and obligate oligomers.

ABSTRACT: Förster resonance energy transfer (FRET) is a nonradiative process for the transfer of energy from an optically excited donor molecule (D) to an acceptor molecule (A) in the ground state. The underlying theory predicting the dependence of the FRET efficiency on the sixth power of the distance between D and A has stood the test of time. In contrast, a comprehensive kinetic-based theory developed recently for FRET efficiencies among multiple donors and acceptors in multimeric arrays has waited for further testing. That theory has been tested in the work described in this article using linked fluorescent proteins located in the cytoplasm and at the plasma membrane of living cells. The cytoplasmic constructs were fused combinations of Cerulean as donor (D), Venus as acceptor (A), and a photo-i

SUBMITTER: Patowary S 

PROVIDER: S-EPMC4390836 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature