Generating customized transgene landing sites and multi-transgene arrays in Drosophila using phiC31 integrase.

ABSTRACT: Transgenesis in numerous eukaryotes has been facilitated by the use of site-specific integrases to stably insert transgenes at predefined genomic positions (landing sites). However, the utility of integrase-mediated transgenesis in any system is constrained by the limited number and variable expression properties of available landing sites. By exploiting the nonstandard recombination activity exhibited by a phiC31 integrase mutant, we developed a rapid and inexpensive method for isolating landing sites that exhibit desired expression properties. Additionally, we devised a simple technique for constructing arrays of transgenes at a single landing site, thereby extending the utility of previously characterized landing sites. Using the fruit fly Drosophila melanogaster, we demonstrate the fea

SUBMITTER: Knapp JM 

PROVIDER: S-EPMC4391550 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature