Chromatin remodeling factors Isw2 and Ino80 regulate checkpoint activity and chromatin structure in S phase.

ABSTRACT: When cells undergo replication stress, proper checkpoint activation and deactivation are critical for genomic stability and cell survival and therefore must be highly regulated. Although mechanisms of checkpoint activation are well studied, mechanisms of checkpoint deactivation are far less understood. Previously, we reported that chromatin remodeling factors Isw2 and Ino80 attenuate the S-phase checkpoint activity in Saccharomyces cerevisiae, especially during recovery from hydroxyurea. In this study, we found that Isw2 and Ino80 have a more pronounced role in attenuating checkpoint activity during late S phase in the presence of methyl methanesulfonate (MMS). We therefore screened for checkpoint factors required for Isw2 and Ino80 checkpoint attenuation in the presence of MMS. Here we de

SUBMITTER: Lee L 

PROVIDER: S-EPMC4391558 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature